Sampling Scale

High spatial heterogeneity of soil influences the diversity of microbes and their function. Spatial heterogeneity in soil microbial communities occurs at many scales, from soil particles (e.g., soil microaggregates) to the plant rhizosphere, to field plots, and to the ecosystem and global levels. In order to improve our knowledge of how microbial community structure influences ecosystem function, we must obtain more quantitative knowledge of the interaction between microbes, plant residues, and soil management at a variety of spatial scales.

summary

We have come a long way in developing our understanding of microbial ecology, but have many milestones yet to meet. Molecular tools offer unparalleled opportunities to characterize microbes in culture and directly from field soils. These tools are allowing us to ask questions at much larger geographic scales than has been possible previously. We are now able to examine such issues as how micro-bial populations vary across soil types and climatic zones, in association with plant roots and between various plant species, and in response to soil management or soil pollution. We are now just seeing the tip of the iceberg in terms of soil microbial diversity with the use of molecular approaches. The amount of work that remains is daunting, yet exciting as so much remains to be discovered. Recent techniques developed for the study of microbial populations, such as T-RFLP and

DGGE, allow access to the very large proportion of organisms that are present in the soil and which remain unculturable under laboratory conditions. Other techniques, such as RFLP analysis of isotopically labeled, amplified nifH, amoA, nirS, and pmoA sequences or SIP will allow us to target, with high specificity, organisms or groups of organisms responsible for specific functions in soil, particularly those involved in key transformations in the C, N, and S nutrient cycles. These types of technical developments open new horizons of research and applications that will allow a far more complete and less biased view of microbial diversity and function in soils.

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