Uncultivated Organisms

Molecular phylogeny was initially based on prokaryotes that could be cultivated in the laboratory, making 16S rRNA gene sequencing relatively easy. However, e'noCl

FIGURE 5.2 The major phylogenetic divisions within the Bacteria determined by analysis of 16S rRNA gene sequences. Unshaded segments represent groups with cultivated representatives, although many of these have very few such representatives. Shaded segments represent sequences obtained by direct amplification of 16S rRNA genes from environmental DNA, without a cultivation step (with permission from Hugenholtz et al., 1998).

Bbrobacter

Marine group A Green sulfur

Cytophaga/es

The e'noCl

FIGURE 5.2 The major phylogenetic divisions within the Bacteria determined by analysis of 16S rRNA gene sequences. Unshaded segments represent groups with cultivated representatives, although many of these have very few such representatives. Shaded segments represent sequences obtained by direct amplification of 16S rRNA genes from environmental DNA, without a cultivation step (with permission from Hugenholtz et al., 1998).

TABLE 5.1 Characteristics of Bacterial Phylogenetic Groups with Cultivated Representatives

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