## Calculations on Percentage of INH and EC50

Calculation of MTIs

Values of INH (%) were used to determine the toxicity of chemicals against V.fischeri DSM 7151. They were calculated from BLI changes of both samples and controls, as follows:

where IC0 and IT0 are the initial BLI values of controls and samples, respectively, and IC, and IT, are the BLI values of controls and samples at exposure time (,) to cell suspension, respectively. The INH(%) values presented here are the averages of at least three independent measurements. The EC50 values are the concentrations corresponding to INH(%) = 50 in the relationship between INH(%) and concentration of chemicals.

MTI values were calculated by the following equation of Konemann:

where Mo = M/fM« and M = f = £ C(i)/LC5o(i). fMax is defined as the largest fi) value in the mixture. C(i) and LC50(i) are the concentrations of ith component in the mixture and its LC50 value, respectively. EC50 values are used instead of LC50 in this article. When MTI values were lower than zero, the mixture potency was defined as antagonism. If they are equal to zero, there was no additive effect. The mixture potency was defined as partially additive when the values were higher than zero but less than 1. When they are equivalent to or greater than 1, the mixture potency was defined as additive or synergistic, respectively.

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