Measurements of BLI

As described in the previous papers of the authors, the cells of V. fischeri DSM 7151 were grown in the LM medium, centrifuged at 10 000 x g for 1 min at 4 °C, and then suspended in ASW. Aliquot 0.2 ml cell suspension of V. fischeri was added into each well of a 96-well microplate. Then, BLI changes in each well containing 0.2 ml of sample or control were followed using a multidetection microplate reader (Powerscan HT, Dainippon Pharmaceutical, Osaka, Japan) during 30 min of incubation.

O S Cu-pt (1:2 complex) Copper 2-pyridinethiol 1-oxide

O S Cu-pt (1:2 complex) Copper 2-pyridinethiol 1-oxide

TPBP

Triphenylborane pyridine

TBT-Cl Tributyltin chloride

Cl C Cl

O CH3

Cl C Cl

N-Dichlorofluoromethylthio-N', N '-dimethyl-N-phenylsulfamide

TPBP

Triphenylborane pyridine

Diuron

SEA-NINE 211

SEA-NINE 211

3-(3,4-Dichlorophenyl)-1,1-dimethylurea 4,5-Dichloro-2-(n-octyl)-3(2H)-isothiazolone

Ziram

Zinc bis(W,W '-dimethyl) dithiocarbamate

Zn-pt (1:2 complex) Zinc 2-pyridinethiol 1-oxide

I C=C COCN CCCCH

IPBC

3-Iodo-2-propynyl butylcarbamate Figure 1 Molecular structures of antifouling chemicals examined.

H3C H3

H3C H3

CH3 N

Irgarol 1051

2-Methylthio-4-fert-butylamino-6-cyclopropylamino-s -triazine

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