Assigning a locality to the respective class is based on a concrete value of the saprobic (also saprobe, saprobial) index. The saprobic index is most frequently expressed by the following formula:
where i is a taxon, n represents the number of taxa, Ai is the abundance of a taxon found or collected, si equals the saprobic value of the taxon in question, and g is the indication weight of the taxon. Saprobic index is a ratio of the sum of products of abundance, saprobic value, and the indication weight of individual species, and the sum of products of their abundance and indication weight.
The values of saprobic index in the limnosaprobity classes are classified in the following ranges:
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