Use and Care of a Microscope

Eyepiece Contains magnifying lenses you look through.

Arm Supports the body tube.

Low-power objective Contains the lens with the lowest power magnification.

Stage clips Hold the microscope_ slide in place.

Coarse adjustment Focuses the image under low power.

Fine adjustment Sharpens the image under high magnification.

Eyepiece And Body Tube

Body tube Connects the eyepiece to the revolving nosepiece.

Revolving nosepiece Holds and turns the objectives into viewing position.

High-power objective Contains the lens with the highest magnification.

Stage Supports the microscope slide.

Light source Provides light that passes upward through the diaphragm, the specimen, and the lenses.

Base Provides support for the microscope.

Caring for a Microscope

1. Always carry the microscope holding the arm with one hand and supporting the base with the other hand.

2. Don't touch the lenses with your fingers.

3. The coarse adjustment knob is used only when looking through the lowest-power objective lens. The fine adjustment knob is used when the high-power objective is in place.

4. Cover the microscope when you store it.

Using a Microscope

1. Place the microscope on a flat surface that is clear of objects. The arm should be toward you.

2. Look through the eyepiece. Adjust the diaphragm so light comes through the opening in the stage.

3. Place a slide on the stage so the specimen is in the field of view. Hold it firmly in place by using the stage clips.

4. Always focus with the coarse adjustment and the low-power objective lens first. After the object is in focus on low power, turn the nosepiece until the high-power objective is in place. Use ONLY the fine adjustment to focus with the high-power objective lens.

Making a Wet-Mount Slide

1. Carefully place the item you want to look at in the center of a clean, glass slide. Make sure the sample is thin enough for light to pass through.

2. Use a dropper to place one or two drops of water on the sample.

3. Hold a clean coverslip by the edges and place it at one edge of the water. Slowly lower the coverslip onto the water until it lies flat.

4. If you have too much water or a lot of air bubbles, touch the edge of a paper towel to the edge of the coverslip to draw off extra water and draw out unwanted air.

Matt Meadows

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