(Precautionary note: Conduct this work in a fume hood.)
Using hot formalin, the nematodes are instantly killed and fixed. Immediately cooling down with cold formalin prevents overheating that could cause deformations. With this hot-cold formalin method, nema-todes will be fixed in taxonomic groups according to characteristic body curvatures, which makes routine identification easier. Another advantage is that most species keep their body transparency. This is in contrast to the method of fixing nematodes with cold formalin only, where nematodes die slowly instead of instantly, which causes deformations and loss of transparency.
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