Figure 3.15 Two-dimensional thin-layer chromatograph separation of amino acids. (Based on White et. al., 1973.)
them. The radioactivity makes compounds easier to detect in small quantities. For example, exposing plants to carbon dioxide with carbon-14, and then analyzing plant matter periodically for radioactive compounds is a way to elucidate the steps in photosynthesis as the 13C appears in one compound and then another. Radioimmunoassay can detect compounds such as hormones or drugs in blood plasma at quantities as low as several picograms (10~12 g).
Other analysis techniques, based on the methods of molecular biology, are discussed in Chapter 6.
Was this article helpful?